You need to get onto the Pune Mumbai Expressway at Pune and get off the Pune Mumbai Expressway at the Khopoli exit after passing Lonavala/ Khandala and get onto the old Poona Bombay road. Approximately 18 km later, you turn right at a place called Chowk where a big green sign over the road asks you to turn right for Matheran. From then on there are regular road signs to guide you. From Chowk to Karjat rail crossing is 9 km, then 12 km to Neral at the tip of the hill. After that the last stretch of the road (About 7 km) is on the ghat which leads you to Dasturi Naka (Last point to which cars are allowed, where there is a car park) at the tip of Matheran